Skip to main content
. 2022 Feb 3;13:805379. doi: 10.3389/fphar.2022.805379

FIGURE 4.

FIGURE 4

HSP70-related protective effects of SHED-CM. (A): Representative fluorescent microscopy images of N2a cells expressing mCherry-SOD1G85R incubated for 24 h with SHED-CM (30%, 50%, and 70%) and the HSP70 inhibitor Pifithrin mu (Pi mu) (1 μM/24 h). Arrow heads show SOD1 aggregates. (B): Quantified data of intracellular SOD1 aggregates are expressed as means ± SEM of three independent experiments. In each experiment, at least 2,000 cells were counted. ###p < 0.001 vs. WT; ***p < 0.001, **p < 0.01, and *p < 0.05 vs. G85R; †p < 0.05 vs. G85R treatment of SHED-CM. Scale bar: 10 µm. (C): mCherry-, mCherry-SOD1WT-, and mCherry-SOD1G85R-expressing N2a cells were differentiated in culture medium (2-mM dbcAMP and 2% FBS) in the presence or absence of SHED-CM (30%, 50%, and 70%/48 h) and the HSP 70 inhibitor (Pi mu) (1 μM/48 h). Cell viability was measured via a CCK-8 assay. Results are presented as means ± SEM of three independent experiments or as a percentage of the “mock” (mock = 100%). ###p < 0.001 vs. WT; ***p < 0.001, **p < 0.01, and *p < 0.05 vs. G85R; †p < 0.05.