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. 2022 Feb 3;13:805379. doi: 10.3389/fphar.2022.805379

FIGURE 6.

FIGURE 6

Neuroprotection of ALS motor neurons using (or treated with) SHED-CM. (A): The scheme of this study. (B and D): Generation of motor neurons from iPSCs derived from ALS patient. Representative images of ALS motor neurons on day 7 and day 14 after treatment with vehicle, ultrafiltered SHED-CM (50%), or Fb-CM (50%). Treatment with SHED-CM increased survival of ALS motor neurons compared with the survival of vehicle-treated ALS motor neurons. Scale bar: 200 µm. (C and E): Cell viability was measured by staining SMI-32. Results are presented as means ± SEM of three independent experiments based on the fluorescence intensity of the “mock” (mock = 100). ###p < 0.001 vs. Day 7; ***p < 0.001 vs. Day 14. Scale bar: 200 µm. (F): Accumulation of misfolded SOD1 protein in ALS patient iPSC-derived motor neurons was detected using an immunoprecipitation assay.