Fig. 5. Effector T cells from Mtb-HT infected mice reduce bacterial burden and inflammation in Mtb-LT infected mice.
Three days prior to infection naïve mice were retro-orbitally injected with PBS or 2 × 106 CD3+ T cells isolated from 6 to 7-week Mtb-LT1 or Mtb-HT1 infected mice. On the day of infection, half of the mice in each group were infected with Mtb-HT1 and the other half with Mtb-LT1 via Glas-Col aerosol exposure. At 11 weeks post-infection, lung bacterial burden was determined and presented as CFU (a) and protein levels of IFNγ (b), TNF (c), IL-17 (d), and IL-1β (e) was measured in lung lysates by ELISA. Data are presented as mean values +/− SD. Source data are provided as a Source Data file. Sample size of n = 5 mice was included in each group. Significance was determined using one-way ANOVA with Tukey’s correction *P = 0.0421 (IFNγ panel b), **P = 0.0013 (IL-17, panel c) *P = 0.0132 (TNF, panel b), ***P < 0.001, ****P < 0.0001. The experiment was repeated with Mtb-HT2 and Mtb-LT2.