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. 2022 Feb 16;13:884. doi: 10.1038/s41467-022-28506-2

Fig. 6. IL-1R dependent migration of alveolar macrophages in Mtb-HT infection. C3HeB/FeJ mice were infected with ~100 CFU of Mtb-LT1 or Mtb-HT1 via Glas-Col aerosol exposure.

Fig. 6

At 13 days post infection, fluorescent-labeled anti-SiglecF antibody was intratracheally administered 30 min prior to sacrifice to stain and sort airway resident cells. Differential gene expression analysis by NanoString was performed on airway-resident AMs (CD11b-CD11c+SiglecF+) sorted from single cell lung suspensions pooled from five Mtb-HT1 and five Mtb-LT1 infected mice and the data are presented to show log fold change (logFC) of top DEG (Y-axis, P < 0.05) (a). For IL-1R dependent AM migration, groups of infected mice were administered intra-peritoneally on days 8, 10, and 12 post infection with either 200 μg α-IL-1R1 antibody or isotype control. Data are presented as airway-resident AMs (CD11b-CD11c+SiglecF+) (b) and AMs migrated into the interstitium (CD11b-CD11c+SiglecF−) (c). Data are presented as mean values +/− SD (panels b and c). Source data are provided as a Source Data file. Sample size of n = 4 mice were included for the first 4 groups and sample size of n = 5 mice for group 5 for panels (b, c). Significance was determined using one-way ANOVA with Tukey’s correction. ****P < 0.0001. The data are representative of one of two individual experiments with Mtb-HT1 and Mtb-LT1 and was repeated with Mtb-HT2 and Mtb-LT2.