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. 2022 Feb 16;13(2):157. doi: 10.1038/s41419-021-04210-9

Fig. 3. LncRNA ROR upregulates ALDH1A1/TESC to promote TPC-1 cell proliferation, migration, and invasion while inhibiting cell apoptosis.

Fig. 3

A The co-expression relationship between TESC and ALDH1A1 from MEM analysis. ALDH1A1 has multiple probes, i.e., multiple co-expression relationships are shown in the figure. B Venn diagram of differentially expressed genes in thyroid cancer samples from the GSE3467, GSE33630, and GSE97001 datasets and co-expression genes predicted using MEM. C PPI network diagram of 10 intersection genes and their related genes constructed by GeneMANIA. The larger the circle for the gene is, the higher the core degree is. D ALDH1A1 expression in PTC tissue and adjacent tissue samples of patients with PTC as detected by immunohistochemistry (×400, n = 85). *p < 0.05 vs. adjacent tissues. E TESC expression after alteration of TESC in TPC-1 cells as determined by RT-qPCR, normalized to GAPDH. *p < 0.05 vs. oe-NC. #p < 0.05 vs. sh-NC. F ALDH1A expression after alteration of TESC in TPC-1 cells as determined by RT-qPCR, normalized to GAPDH. *p < 0.05 vs. oe-NC. #p < 0.05 vs. sh-NC. G ALDH1A protein expression after alteration of TESC in TPC-1 cells as determined by western blot analysis, normalized to GAPDH. *p < 0.05 vs. oe-NC. #p < 0.05 vs. sh-NC. H ALDH1A1 expression after ALDH1A1 alteration and TESC overexpression in TPC-1 cells as determined by RT-qPCR. I TPC-1 cell viability after ALDH1A1 alteration and TESC overexpression, as detected by MTT. J Colony formation of TPC-1 cells after ALDH1A1 alteration and TESC overexpression, as detected by colony formation assay. K TPC-1 cell migration and invasion after ALDH1A1 alteration and TESC overexpression as detected by Transwell assay (×200). L TPC-1 cell apoptosis after ALDH1A1 alteration and TESC overexpression as detected by flow cytometry. In panel H–L, *p < 0.05 vs. oe-NC, #p < 0.05 vs. sh-NC, &p < 0.05 vs. oe-TESC. M The expression of ALDH1A1 in TPC-1 cells transduced with oe-lncRNA ROR + sh-TESC-1 or sh-ALDH1A1-1, as determined by RT-qPCR, normalized to GAPDH. N TPC-1 cell viability after transduction with oe-lncRNA ROR + sh-TESC-1 or sh-ALDH1A1-1, as detected by MTT. O Colony formation of TPC-1 cells after transduction with oe-lncRNA ROR + sh-TESC-1 or sh-ALDH1A1-1, as detected by colony formation assay. P TPC-1 cell migration and invasion after transduction with oe-lncRNA ROR + sh-TESC-1 or sh-ALDH1A1-1 as detected by Transwell assay (×200). Q TPC-1 cell apoptosis after transduction with oe-lncRNA ROR + sh-TESC-1 or sh-ALDH1A1-1 as detected by flow cytometry. In panel M–Q, *p < 0.05 vs. oe-lncRNA ROR + sh-NC. Cell experiments were repeated three times independently.