Fig. 2. Activity of individual ROIs and their functional clusters during habituation.

a Responses of ROIs across the brain to a loom stimulus, color-coded for the normalized intensity of their response. b, c The same ROIs’ responses to the second and tenth looms. d, e The degree of habituation in each of these ROIs in the second and 10th trials, calculated as the ratio of response to the first loom. This analysis was restricted to ROIs showing clear responses (with a coefficient of determination (r² value) > 0.5 for the linear regression between their response and a regressor simulating a calcium signal) for the first loom stimulus. Raster plots (f) and mean response traces (g) of the ROIs composing each of five functional clusters, with a clear correspondence to the three blocks of ten stimuli. The x axis scale at the bottom of g also applies to f. h Anatomical locations for the ROIs belonging to each functional cluster (colors matching the mean traces in g). Since different animals startled in different trials, we identified the motor cluster using a different regressor for each animal. The mean responses are shown for a single animal in this cluster in g, with yellow lines indicating the relevant neurons from that animal in f. A rotation of h can be found in Supplementary Movie 1, and the distributions of these clusters are detailed in virtual sections in Supplementary Fig. 5. Data shows the pooled responses of 11 larvae to the f20 stimulus train. Relevant anatomical brain regions are indicated in the bottom right corner of h, each shown for only one side of the brain. Pallium, Pal; subpallium, Sp; thalamus, Th; habenula, Hb; pretectum, Pt; tectum, Tec; tegmentum, Tg; cerebellum, Cb; and hindbrain, HB. R, rostral; C, caudal.