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. 2022 Feb 16;12:2573. doi: 10.1038/s41598-022-06229-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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NLRP3 inflammasome dependent secretion of IL-1β and IL-18 in human PBMCs infected with SFTSV. Effect of NLR knockdown by shRNA on the processing of pro-caspase-1 and secretion of IL-1β was shown in PBMCs. The cells were infected with the lentiviruses which expressed shRNA specifically for knockdown of NLRP1, NLRP3, NLRC4 or AIM2, prior to be infected with SFTSV. (A,B) Secretion of IL-1β and IL-18 were measured in the culture medium of PBMCs infected with or without SFTSV by ELISA. (C) Knockdown of NLRs and cleavage of pro-caspase-1 in PBMCs infected with or without SFTSV detected by western blot with antibodies specific for respective proteins. (D) Comparable effect of shRNA on the viability of PBMCs as determined with a CCK-8 assay. ***P < 0.001. P-values were calculated using a One-way ANOVA test.