Figure 6.

Inhibition of NLRP3 and caspase-1 reduced induction of membrane damage and cell death in PBMCs. PBMCs were pre-treated with DMSO alone, glibenclamide, or Ac-YVAD-cmk for 6 h prior to viral infection at an MOI of 1 for 48 h. The cells were subjected to staining with Calcein-AM/EthD-III for detection of membrane damage and cell death, which were analyzed by Flow cytometry. (A) DMSO alone plus SFTSV; (B) glibenclamide (1 μM) plus SFTSV; (C) Ac-YVAD-cmk (1 μM) plus SFTSV; and (D) DMSO. The ratio of membrane damage cells was determined using flow cytometry and differences between groups were analyzed by chi-square test. (E) Processing of GSDMD in PBMCs infected with SFTSV. The cells were with SFTSV an MOI of 1 and cell lysates were prepared at various time points p.i. for SDS-PAGE and western blot analysis with antibodies specific for GSDMD and GSDMD-N.