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. 2022 Feb 16;12:2651. doi: 10.1038/s41598-022-06588-8

Figure 5.

Figure 5

Myelin gene expression in sciatic nerves of Pbrm1 cKO mice. (A) Quantitative RT-PCR of Mbp and Mpz expression in sciatic nerves of control (Ctrl) and Pbrm1 cKO mice from P7 until P60 (shown as mean ± SEM, n = 3 per genotype). Average expression levels for each marker at each analyzed time point were set to 1. (B, C) Determination of Mbp-positive structures (B) and Mpz-expressing cells (C) per 1000 square micrometers in sciatic nerve sections of control and Pbrm1 cKO mice from P0 until P60 (shown as mean ± SEM, n = 3 per genotype). (D, E) Immunohistochemical staining for Mbp protein (D) and in situ hybridization with an Mpz-specific antisense riboprobe (E) of nerve sections from control and Pbrm1 cKO mice between P0 and P60. Scale bars: 10 µm (D), 500 µm (E). Statistical significance between genotypes was determined separately for each time point and marker by unpaired two-tailed Student’s t-test. However, no significant difference was detected.