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. 2022 Feb 16;13(2):156. doi: 10.1038/s41419-022-04602-5

Fig. 5. P32 interacts with CHCHD2 and CHCHD10 to regulate mitochondrial morphology and mtISR.

Fig. 5

A Hela cell lysates were immunoprecipitated with anti-P32 antibody or IgG, followed by immunoblotting using the indicated antibodies. B Lysates of P32-knockdown-Hela cells treated with DMSO or CCCP (10 uM, 124 h) analyzed for indicated protein expression by immunoblot. C Representative immunofluorescence images of mitochondrial morphology in control, shP32 Hela cells (mean ± s.d. of n = 3 independent biological samples; two-way ANOVA with Tukey’s multiple comparisons correction, ***P < 0.001). D Mitochondrial morphology described in C was counted according to the criteria detailed in Experimental Procedures. E Representative western blots of the indicated proteins in WT and shP32 Hela cells after 10uM CCCP treatment for 24 h. F Representative electron microscopic images and quantification of mitochondrial ultrastructure in WT or shP32 Hela cells.