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. 2022 Feb 16;12:2637. doi: 10.1038/s41598-022-06700-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunolocalization of docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA) and arachidonic acid (ARA) in testis and cauda epididymis of rabbits fed control and FLAX diets. In (a) a clear DHA localization was in interstitial tissue, Sertoli cells and spermatogonia; after FLAX diet (d), the signal was also evident in elongated spermatids (arrow); in (b) the EPA label appeared localized in the germ cells at different stages of maturation; after FLAX diet, the label was intense in the same cells (e); in (c) the ARA signal was detected in interstitial cells, Sertoli cells (arrow) and elongated spermatids of control testis. The same labelling was present in testis from rabbits fed FLAX diet (f). In the epididymis of rabbits fed control (g,h,i) and FLAX diets (j,k,l), in (g,i,j and l), a limited number of vesicles in the lumen were labelled (DHA, g and j; ARA, i and l); on the contrary, in (h,k) a number of vesicles appeared labelled in the lumen where the spermatozoa were located (EPA). Bar: a–f, 10 µm, g–l, 50 µm.