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Determination of the cytotoxicity in B16 cells. Cells were treated with (A) MC (B) GMC (C) MR (D) GMR for 3 days. And then, the cell viability was assessed using a CCK-8 assay. Absorbance was measured using a microplate reader (470 nm). Bar graph (mean ± SEM) data from three experiments were determined using one-way ANOVA with Tukey's post hoc test, ∗∗∗P < 0.001; ∗∗P < 0.01; ∗P < 0.05, compared with the vehicle group.
