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. 2022 Feb 3;13:830763. doi: 10.3389/fphar.2022.830763

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Copyright © 2022 Han, Wang, Wang, Qiao, Cui, Zhang, Jiang, Liu, Shangguan, Zheng, Bai, Du and Shen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DBZ inhibited stress-induced autophagy. (A–D) Representative Western blotting assay and quantification of LC3, p62, and Beclin1 expression. n = 3. (E) Immunofluorescence staining of p62 in cardiac tissues. Scale bars = 50 μm. n = 3. (F) Representative electron micrographs of autophagic vacuoles in cardiomyocytes of rats with various treatments. Scale bars = 2 μm. n = 3. (G,H) Immunofluorescence staining and quantification of LC3-II in NRCMs. n = 5. Scale bars = 20 μm. (I) Representative Western blotting assay of Atg7, p62, Beclin1, Atg5 and LC3 expression. Results are expressed as means ± SD. Statistical analyses were performed by one-way ANOVA followed by Bonferroni’s post-hoc test.