FIGURE 8.

Nrf2 activation was critical for the antioxidant and inhibiting ER stress of DBZ. (A,B) Representative Western blotting assay and quantification of nucleosol Nrf2 expression with DBZ treatment. n = 4. (C–E) Representative Western blotting assay and quantification of nucleosol and total Nrf2 expression. n = 3. (F,G) Cells transfected with Nrf2 siRNA or NC siRNA, protein levels of nucleosol Nrf2 were determined by Western blotting assay and quantified by densitometry. n = 5. (H) Representative Western blotting assay of PERK, p-PERK, GRP78, XBP1 and CHOP expression. *p < .05 compared with the Control group, **p < .05 compared with the Ang II group, ***p < .05 compared with the Ang II + DBZ group. Results are expressed as means ± SD. Statistical analyses were performed by one-way ANOVA followed by Bonferroni’s post-hoc test.