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. 2022 Feb 3;8:795747. doi: 10.3389/fcvm.2021.795747

Figure 3.

Figure 3

AOPP-BSA mediates endothelial hyperpermeability and impairs angiogenesis. (A,C) HUVECs were stimulated with 200 μg/ml AOPP-BSA for 0, 1, 3, 6, 12, 24 h, then transendothelial electrical resistance (TER) and permeability coefficient of FITC-dextran (Pa) were detected (n = 4). (B,D) HUVECs were treated with AOPP-BSA at 0~400 μg/ml for 12 h, then TER (n = 3) and Pa (n = 4) were measured. (E,F) CCK-8 assay was used to evaluate the proliferation function of HUVECs with AOPP-BSA treatment for different time (n = 4) or at different concentrations (n = 5). (G,I) The migration function of HUVECs treated with AOPP-BSA was detected by trans-well migration assay. Scale bar = 50 μm. (H,J) The number of trans-well migration cells was counted (n = 3–5). (K,N) Matrigel tube formation assay was used for tube formation evaluation in HUVECs incubated with AOPP-BSA. Scale bar = 30 μm. (L,M) Tube length and branching points of HUVECs treated with AOPP-BSA for different time were quantitated (n = 5). (O,P) Tube length and branching points of HUVECs treated with AOPP-BSA at different concentrations were quantitated (n = 3). Two-way ANOVA was used for statistical analysis. All data are shown as mean ± SEM. ns, non-significant (P > 0.05).