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. 2022 Feb 3;8:795747. doi: 10.3389/fcvm.2021.795747

Figure 7.

Figure 7

Autophagy inhibition enhances p53 SUMOylation induced by AOPP-BSA. (A,C) HUVECs were incubated with 200 μg/ml AOPP-BSA for 12 h. Effects of AOPP-BSA on p53 and p53 SUMOylation were detected by western blot and immunoprecipitation. (B) Quantification of p53 protein expression (n = 4). (D) The ratio of SUMOylated p53 and total p53 was calculated (n = 4). (E,G) HUVECs were pretreated with Rapa (200 nM) or 3-MA (5 mM) for 2 h before AOPP-BSA application, and the levels of p53 and p53 SUMOylation were detected by western blot and immunoprecipitation. (F) Quantification of p53 protein expression (n = 4). (H) The ratio of SUMOylated p53 and total p53 was calculated (n = 3). Two-tailed unpaired Student's t-test for (B). One-way ANOVA with LSD or Dunnett's T3 post-hoc multiple comparisons for (D,F,H). Data are shown as mean ± SEM. ns, non-significant (P > 0.05). SUMO1, small ubiquitin related modifier 1; SUMO1-p53, SUMOylated p53; IP, immunoprecipitation; IB, immunoblotting.