Fig. 3.

SELENBP1 inhibits aggressiveness of CRC cells and HUVECs by blocking intercellular communications. HUVECs and HCT-15 cells were transduced with lentiviruses carrying SELENBP1, shSELENBP1, or respective controls to prepare conditional medium. HCT-15 and HCT-116 cells were cultured in CMs from HUVECs and underwent migration and invasion assays (A and B). HUVECs were cultured in CMs from HCT-15 cells and underwent migration, invasion, and tube formation assays (C). Abbreviations: CM, conditional medium; HUVEC, human umbilical vascular endothelial cell; SELENBP1, selenium binding protein 1. *p < 0.05; ^⁎⁎p < 0.01; ^⁎⁎⁎p < 0.001; ^⁎⁎⁎⁎p < 0.0001 vs. the control.