REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-ZO-1 antibody	Thermo Fisher Scientific	cat. # 33-9100; RRID:AB_2533147
goat anti-mouse IgG antibody, Alexa Fluor 488	Thermo Fisher Scientific	cat. # A28175; RRID:AB_2536161
anti-Claudin-1 antibody	Thermo Fisher Scientific	cat. # 51-9000; RRID: AB_2533916
goat anti-rabbit IgG antibody, Alexa Fluor 555	Thermo Fisher Scientific	cat. # A27039; RRID:AB_ 2536100
Chemicals, peptides, and recombinant proteins
Blocking One	Nacalai Tesque	cat.# 03953
KSOM	Millipore	cat. # MR-106D
Ouabain	Sigma-Aldrich	cat.# O3125
Alexa Fluor 546 Phalloidin	Thermo Fisher Scientific	cat.# A22283; RRID:AB_2632953
Hoechst 33342	Thermo Fisher Scientific	cat.# H3570
Lipofectamine 2000	Invitrogen	cat. # 11668-027
Effectene	Qiagen	cat. # 301425
Latrunculin B	Enzo Life Science	cat. # BML-T110-0001
Digitonin	WAKO	cat. # 048-02124
Trypsin	Nacalai Tesque	cat # 35555-54
(3-aminopropyl) triethoxysilane	Sigma-Aldrich	cat. # 440140
Sulfo-SANPAH	Pierce	cat. # 22589
1.6-hexanediol	WAKO	cat. # 087-00432
Experimental models: organisms/strains
R26-ZO-1EGFP	RIKEN	accession # CDB026K
wild type ICR mice	Japan CREA	N/A
Experimental models: cell lines
MDCK I cells	Fujiwara et al. (2016)	N/A
A6 cells	Mimori-Kiyosue et al. (2007)	N/A
Recombinant DNA
human ZO-1	Konno et al. (2008)	N/A
GFP-ZO-1/pCS2	This paper	N/A
GFP-ZO-1ΔABD/pCS2 (Δ1151-1371)	This paper	N/A
GFP-ZO-1ΔABD-ΔIDR123/pCS2 (Δ114-118, Δ275-415, Δ588-627, Δ1151-1371)	This paper	N/A
GFP-ZO-1ΔIDR4/pCS2 (Δ858-1580)	This paper	N/A
GFP-ZO-1-PSG/pCS2 (424-794)	This paper	N/A
GFP-ZO-1ΔABDΔPSG/pCS2 (Δ424--794, Δ1151-1371)	This paper	N/A
Lifeact-RFP	Iioka et al. (2007)	N/A
RFP-MoesinABD	Iioka et al. (2004)	N/A
mCherry-Utrophin	Kim and Davidson (2013)	N/A
Software and algorithms
Fiji	NIH	https://fiji.sc
Python 3.9	Python Core Team	https://www.python.org
Pandas	ver. 1.2.4	http://pandas.pydata.org
Seaborn	ver. 0.11.1	https://seaborn.pydata.org
Matplotlib	ver. 3.4.2	https://matplotlib.org
scipy.stats	ver. 1.6.3	https://docs.scipy.org/doc/scipy/reference/stats.html
statsmodels.stats.multicomp	ver. 0.13.1	https://www.statsmodels.org/dev/index.html
Andor IQ2	Oxford Instruments	https://andor.oxinst.com/products/iq-live-cell-imaging-software/
PrDOS (protein disorder prediction system)	Ishida and Kinoshita (2007)	http://prdos.hgc.jp/cgi-bin/top.cgi