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. 2022 Feb 3;14:807090. doi: 10.3389/fnmol.2021.807090

FIGURE 4.

FIGURE 4

Disinhibition of PV-INs upon disruption of KCC2. (A) Position of stimulus and recording electrodes for field recordings in the stratum pyramidale and radiatum of CA1. (B) Paired-pulse ratios are reduced in the stratum pyramidale (n = 28/24 cells from N = 9/9 mice), but not in the stratum radiatum (n = 35/34 cells from N = 11/11 mice) of KCC2 KOPV mice (2-way ANOVA repeated measure; Bonferroni post-test; ***p < 0.0001). (C) Representative examples of fESPs recorded in the stratum radiatum and stratum pyramidale in KCC2 WTPV and KCC2 KOPV mice. (D) E-S-coupling is diminished in 8-week-old KCC2 KOPV mice (n = 14/17 slices from N = 4/4 mice, 2-way ANOVA repeated measure; Bonferroni post-test; **p = 0.008; ***p < 0.0001). (E) The frequency, but not the amplitude of spontaneous inhibitory post synaptic currents (sIPSCs) is increased in CA1 pyramidal neurons in KCC2 KOPV mice (N = 6/6 mice, unpaired Student’s t-test; Kolmogorov-Smirnov test; ns not significant; *p = 0.0398). (F) Miniature inhibitory postsynaptic currents (mIPSCs) upon tetrodotoxin (TTX) inhibition of spontaneous network driven events are not changed in 8-week-old KCC2 KOPV mice (N = 6/6 mice; unpaired Student’s t-test; Kolmogorov-Smirnov test; ns not significant).