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. 2022 Feb 3;12:830343. doi: 10.3389/fpls.2021.830343

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Copyright © 2022 Yin, Wei, Han, Chen, Gao and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A phylogenetic tree and enzymatic analysis to screen for flavonol glucosyltransferases. (A) A phylogenetic tree of 30 AcUGTs and reported UGTs, sequence of reported UGTs were from Yin et al. (2021). (B) The UPLC spectra of AcUGT recombinant proteins with UDPG and kaempferol (Ka). (C) The UPLC spectra of AcUGT recombinant proteins with UDPG and quercetin (Qu). The green arrows indicate substrates, while the red arrows indicate products. KG STD, astragalin standard; IQG STD, isoquercitrin standard. CK means control, the enzymatic activity of the boiled protein encoded by empty-vector, substrates and UDP-glucose.