Figure 5.

Production of pro-inflammatory cytokine IL-6 and chemokine MCP-1 by LPS-stimulated macrophages and monocytes upon treatment with ALK1-Fc or control-Fc. Monocytes (n = 3 experiments) and macrophages (n = 3) treated with 0, 10, or 100 ng/ml ALK1-Fc or control-Fc subsequently stimulated with 10 ng/ml LPS. The IL-6 (A,C) and MCP-1 (B,D) concentrations were determined in the supernatant. Statistical evaluation was performed with the unpaired parametric T-test, *p < 0.05, **p < 0.01, ***p < 0.001. n.s. = non-significant.