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. Author manuscript; available in PMC: 2023 May 1.
Published in final edited form as: Transplantation. 2021 Aug 11;106(5):950–962. doi: 10.1097/TP.0000000000003920

Figure 1. Assessment of xenospecific memory T cell responses.

Figure 1.

(a) Human PBMCs were stimulated with PEC monolayers in the presence of protein transporter followed by ICCS to detect cytokine producers. T cells were analyzed based on CD3, CD4, and CD8 expression (top panel). The percentage of single and dual cytokine produces in PEC-stimulated CD4+ and CD8+ cells (bottom) is similar to unstimulated cells. (b) Human PBMCs were stimulated by PECs or HECs. CD8+ cells were interrogated for TNF-a and IFN-γ production. Top panel shows a representative sample demonstrating dual cytokine producers following stimulation with HECs but not xenogeneic PECs. The dual cytokine producers were examined for CD57 and CD28 expression. Three individuals demonstrated detectable rapid memory T cell–mediated immune responses to three primary HECs but lacked immune responses to PECs (bottom left). Allo-specific dual cytokine producers are largely CD57-expressing cells but lack CD28 expression.