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. Author manuscript; available in PMC: 2023 May 1.

Published in final edited form as: Transplantation. 2021 Aug 11;106(5):950–962. doi: 10.1097/TP.0000000000003920

Figure 5. — (a) PBMCs were analyzed for mean fluorescence intensity (MFI) of CD2 expression following PEC stimulation. Histogram of representative samples (left) shows CD2 MFI on proliferating CD4⁺ and CD8⁺ cells (black) when compared with resting (light gray) and nonproliferating (dark gray) cells. The MFI of CD2 on proliferating CD4⁺ but not CD8⁺ cells from all tested individuals (right) is significantly higher than resting and nonproliferating cells. (b) Purified naïve and memory cells in the presence or absence of autologous CD14⁺ monocytes were assessed for MFI of CD2 expression following stimulation with PEC monolayers. (c) CD4⁺ and CD8⁺ cells were segregated into four subsets based on surface CD2 and CD28 expression (left). CD4⁺ proliferating cells show significantly higher levels of CD2^hiCD28⁺ subpopulation when compared with resting and nonproliferating cells. The proliferating CD8⁺ cells are predominantly CD2^hiCD28⁻ and CD2^hiCD28⁺ subsets. (d) Proliferating cells in PEC-stimulated naive and memory cells in the presence or absence of autologous CD14⁺ monocytes showed increasing frequency of CD2^hiCD28⁺ cells. (* p<0.05, ** p<0.001, *** p<0.0001, ****p<0.00001)

Figure 5. — (a) PBMCs were analyzed for mean fluorescence intensity (MFI) of CD2 expression following PEC stimulation. Histogram of representative samples (left) shows CD2 MFI on proliferating CD4⁺ and CD8⁺ cells (black) when compared with resting (light gray) and nonproliferating (dark gray) cells. The MFI of CD2 on proliferating CD4⁺ but not CD8⁺ cells from all tested individuals (right) is significantly higher than resting and nonproliferating cells. (b) Purified naïve and memory cells in the presence or absence of autologous CD14⁺ monocytes were assessed for MFI of CD2 expression following stimulation with PEC monolayers. (c) CD4⁺ and CD8⁺ cells were segregated into four subsets based on surface CD2 and CD28 expression (left). CD4⁺ proliferating cells show significantly higher levels of CD2^hiCD28⁺ subpopulation when compared with resting and nonproliferating cells. The proliferating CD8⁺ cells are predominantly CD2^hiCD28⁻ and CD2^hiCD28⁺ subsets. (d) Proliferating cells in PEC-stimulated naive and memory cells in the presence or absence of autologous CD14⁺ monocytes showed increasing frequency of CD2^hiCD28⁺ cells. (* p<0.05, ** p<0.001, *** p<0.0001, ****p<0.00001)