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. Author manuscript; available in PMC: 2023 May 1.

Published in final edited form as: Transplantation. 2021 Aug 11;106(5):950–962. doi: 10.1097/TP.0000000000003920

Figure 6. — (a) CD4⁺ and CD8⁺ cells are segregated into four subsets based on surface expression of PD-1 and CD57. (b) Both CD4⁺ and CD8⁺ cells significantly upregulate PD-1but not CD57 expression following stimulation by PECs. (c) Purified naïve and memory cells were analyzed for PD-1 expression following induction of proliferative responses by PECs. An increased PD-1 expression on proliferating cells was detected in CD4⁺ and CD8⁺ cells. (d) A representative sample shows that PD-1–specific antibody completely blocks PD-1 expression on CD4⁺ and CD8⁺ cells. (e) VPD-450–labeled responder PBMCs were stimulated with PEC in the absence or presence of PD-1 specific antibody for 6 days. A representative from one individual showed the xenoreactive T cell proliferation with or without PD-1 specific antibody (left). The blockade of PD-1 expression with PD-1 specific antibody is not effective to enhance xenoreactive T cell proliferation in response to PEC. (* p<0.05, ** p<0.001, *** p<0.0001, ****p<0.00001)

Figure 6. — (a) CD4⁺ and CD8⁺ cells are segregated into four subsets based on surface expression of PD-1 and CD57. (b) Both CD4⁺ and CD8⁺ cells significantly upregulate PD-1but not CD57 expression following stimulation by PECs. (c) Purified naïve and memory cells were analyzed for PD-1 expression following induction of proliferative responses by PECs. An increased PD-1 expression on proliferating cells was detected in CD4⁺ and CD8⁺ cells. (d) A representative sample shows that PD-1–specific antibody completely blocks PD-1 expression on CD4⁺ and CD8⁺ cells. (e) VPD-450–labeled responder PBMCs were stimulated with PEC in the absence or presence of PD-1 specific antibody for 6 days. A representative from one individual showed the xenoreactive T cell proliferation with or without PD-1 specific antibody (left). The blockade of PD-1 expression with PD-1 specific antibody is not effective to enhance xenoreactive T cell proliferation in response to PEC. (* p<0.05, ** p<0.001, *** p<0.0001, ****p<0.00001)