FIGURE 6.

Expression validation of 16 selected DEGs. The relative expression levels of 16 DEGs were determined using qRT-PCR at 4°C and −4°C. There were 14 upregulated genes and two downregulated genes, with three biological replications and three technical replications for each. Relative expression levels were calculated by log₂ 2^–ΔΔCt method and log₂FC (RNA-seq). Here, Y-axis indicates relative expression levels and X-axis indicates each comparison group (XMCK-XMT1, XMCK-XMT2, YNCK-YNT1, YNCK-YNT2). An empty histogram indicates either RNA-seq could not detect the gene under this treatment or its expression level was low by | log₂(foldchange)| < 1. XMCK, Xinmai26 was treated at 16°C; XMT1, Xinmai26 was treated at 4°C for 4 h; XMT2, Xinmai26 was treated at −4°C for 4 h; YNCK, Yannong19 was treated at 16°C; YNT1, Yannong19 was treated at 4°C for 4 h; YNT2, Yannong19 was treated at −4°C for 4 h.