Figure 5.

Mean diffusivity and fractional anisotropy at baseline and 4 weeks post-dosing

MR images were acquired from (A) control and (B) AAV9.hCLN2-AEX (1.1 × 10¹⁴ GC/animal) or fractional anisotropy from (C) control and (D) AAV9.hCLN2-AEX (1.1 × 10¹⁴ GC/animal) at two time points: baseline (before dosing, labeled as week 1) and 4 weeks post-dosing with a 3 T Phillips Achieva MRI scanner with Flex L and spine radiofrequency (RF) coils. Pulse sequences were qualitatively reviewed by a board-certified veterinary radiologist and appropriate sequences selected based on spatial resolution and image contrast for region of interest (ROI) creation and signal intensity measurement. Images selected include DTI high iso for mean diffusivity. Statistical analyses were performed, comparing groups against their baseline (Tables S18–S25). A p < 0.05 was seen for mean diffusivity at L6 in AAV9.hCLN2-treated animals.