Figure 7.

s2 neuro T2-TFE signal intensity and STIR signal intensity at baseline and 4 weeks post-dosing

MR images were acquired for s2 neuro T2-turbo field echo (TFE) from (A) control and (B) AAV9.hCLN2-AEX (1.1 × 10¹⁴ GC/animal) or 3D STIR from (C) control and (D) AAV9.hCLN2-AEX (1.1 × 10¹⁴ GC/animal) at two time points: baseline (before dosing, labeled as week 1) and 4 weeks post-dosing with a 3 T Phillips Achieva MRI scanner with Flex L and spine RF coils. Pulse sequences were qualitatively reviewed by a board-certified veterinary radiologist and appropriate sequences selected based on spatial resolution and image contrast for ROI creation and for assessment of s2 neuro T2-TFE or 3D STIR images. Statistical analyses were performed comparing groups against their baseline (Tables S18–S25). A p < 0.05 was seen for s2 neuro T2-TFE signal intensity at L6 and STIR signal intensity at L3, L4, L5, L6, and S1 in AAV9.hCLN2-treated animals.