scRNA-seq of the mediobasal hypothalamus reveals its responseto KPC-induced cachexia. Food intake changes in KPC-induced cachexia over a period of 20 days post implantation (A). Unsupervised clustering of cells from sham and TB mice combined yields 20 different clusters as shown with a tSNE-plot (B). Classification of cell types was performed by identifying key marker genes. Dotplot showing the expression of cluster-specific genes (C). MBH cells from sham and TB mice show identical clustering pattern as shown with a tSNE plot (D). Differentially expressed (DE) genes between sham and TB mice in bulk as shown with a volcanoplot (E). qPCR analysis for stress related genes Mt1, Mt2, Cirbp, Lrrc8a and Aldoc (F). ISH for Mt1 (G) and Aldoc (H) in the MBH for sham and TB mice and the quantification is shown on the right. Scale bar = 100 μm. Graph values represent the mean ± SEM of at least three independent experiments and statistical significance between groups was determined with a Student's t-test (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).