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. 2022 Jan 29;25(2):103833. doi: 10.1016/j.isci.2022.103833

Figure 2.

Figure 2

Abi1 regulates HASM proliferation

(A) Protein expression of control, stable Abi1 knockdown (KD), and rescue cell cultures from non-asthmatic HASM was evaluated by immunoblotting. Abi1 shRNA inhibits Abi1 protein expression, which is restored by rescue. Data are means ± SEM. n = 5. One-way ANOVA was used for statistical analysis.

(B and C) Abi1 knockdown (KD) and rescue affect HASM cell numbers (B) and BrdU incorporation (C). Data are normalized to numbers of cells treated with control (Ctrl) shRNA or microplate readings of cells treated with Ctrl shRNA. Data are means ± SEM. n = 5. One-way ANOVA was used for statistical analysis.

(D) Abi1 overexpression in HASM cells. Data are means ± SEM. n = 5. The t test was used for statistical analysis.

(E and F) The overexpression (OE) of Abi1 enhances HASM cell numbers (E) and (F). Data are means ± SEM. n = 5. The t test was used for statistical analysis. ∗p < 0.05; ∗∗p < 0.01. Data normalization is calculated as follows: Data of treated samples/Data of control samples.