Figure 5.

PDGF induces the spatial redistribution of Abi1 in smooth muscle cells

(A) Jak2 is located on the edge of unstimulated HASM cells. PDGF treatment does not affect Jak2 localization. However, Abi1 translocates to the cell periphery upon PDGF stimulation. The arrows indicate a single line scan to analyze the fluorescence intensity for each cell. The inset plots represent fluorescence intensity of the line scan indicated by the arrows. Data are mean ± SEM (n = 32 cells from three independent experiments). The t test was used for statistical analysis.

(B) The cellular localization of Abi2 is not altered in response to PDGF treatment. Data are mean ± SEM (n = 33 cells from three independent experiments). The t test was used for statistical analysis. ∗p < 0.05. NS, not significant

(C) Abi1 KD does not affect the spatial distribution of Jak2 and PDGF receptor β. Data are mean ± SEM (n = 33–34 cells from three independent experiments). The t test was used for statistical analysis. NS, not significant. Protein distribution was analyzed using the methods described in method details. The relative intensity ratios (periphery/interior) are calculated using the following formula: ratio of each cell/average ratios of control cells. Scale bar, 10 μm