REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Abi1	Sigma	#A5106-200UL, L/N 076M4842V:RRID:AB_2220843
GAPDH	Santa Cruz Biotech	#sc-32233, K0315: RRID:AB_627679
JAK2	Santa Cruz Biotech	#sc-294, G1414:RRID:AB_631854
JAK2	Thermo	AHO1352, QC216934: RRID:AB_2536334
phospho-Jak2 (Y1007/Y1008)	Cell Signaling	#3771S/10: RRID:AB_33040
phospho-Jak1 (Y1034/Y1035)	Cell Signaling	#74129S/2: RRID:AB_2799851
Jak1	Cell Signaling	#50996/1: RRID:AB_2716281
phospho-Jak3 (Y980/Y981)	Cell Signaling	#5031S/7: RRID:AB_10612243
Jak3	Cell Signaling	#5481/1: RRID:AB_10623287
phospho-Tyk2 ((Y1054/Y1055)	Cell Signaling	#68790S/1: RRID:AB_2799752
Tyk2	Santa Cruz	#SC-5271/C1221: RRID:AB_628419
phospho-STAT1 (Y701)	Cell Signaling	#9167S/25: RRID:AB_561284
STAT1	Cell Signaling	#9176S/8: RRID:AB_2240087
phospho-STAT2 (Y960)	Cell Signaling	#88410S/4: RRID:AB_2800123
STAT2	Cell Signaling	#4597S/2: RRID:AB_2198305
phospho-STAT3 (Y705)	Cell Signaling	#9145/1: RRID:AB_2491009
STAT3	Invitrogen	#MA1-13042/PJ208446: RRID:AB_10985240
phospho-PDGFRβ (Tyr751)	Cell Signaling	#3161/7: RRID:AB_331053
PDGFRβ	Proteintech	#13449-1-AP/00070807: RRID:AB_2162644
Goat anti-Mouse 488 Alexa Fluor 488	Life Technologies	A11001/1664729: RRID:AB_2534069
Goat anti-Rabbit Alexa Fluor 488	Life Technologies	A11008/1622775: RRID:AB_143165
Goat anti-rabbit Alexa Fluor 546	Life Technologies	A11010/1600212: RRID:AB_2534077
Goat anti-Mouse Alexa Fluor 555	Life Technologies	A21422/1608465: RRID:AB_141822
Bacterial and virus strains
BL21DE3plysS	Promega	L1195
Dh5α	ThermoFisher	18258012
Stbl3	ThermoFisher	C737303
One Shot Top10	ThermoFisher	C404010
Lentivirus encoding STAT3 luciferase promoter	BPS Bioscience	#79744
Lentivirus encoding Abi1 shRNA	Santa Cruz	sc-40306-V
Lentivirus encoding control shRNA	Santa Cruz	sc-108080
Biological samples
Primary human airway smooth muscle cells	This article	N/A
Chemicals, peptides, and recombinant proteins
Ham’s F-12 nutrient mix	GIBCO	11765-047/2187149
Protein A/G Plus-Agarose	Santa Cruz Biotech.	SC-2003
PDGF-BB	Sigma	# P3201-10UG Lot# SLBW2507 & Lot# SLBZ6214
SDS	Bio-Rad	161-0302
Human STAT3	Abcam	Ab268982/GR3352364-3
Trypan blue	Bio-Rad	145-0013
DAPI	Abnova	U0031/K004-HADBI
Triton-X-100	Fisher	BP151-500
Critical commercial assays
HiPure Plasmid Maxiprep Kit	ThermoFisher	K210007
BrdU Cell Proliferation Kit	Millipore Sigma	2750
STAT3 luciferase reporter	BPS Bioscience	#79744
Abi1 ELISA kit	Mybiosource	MBS2018864
Kinase-Glo kinase assay	Promega	V6711
Experimental models: Cell lines
Primary human airway smooth muscle cells	This article	N/A
Experimental models: Organisms/strains
Abi1smko mouse	This article	N/A
Oligonucleotides
Primer for Abi1 forward 5′-GAGCGCCCTGTAAGGTATATT-3′	This article	N/A
Primer for Abi1 reverse 5′-GTTCTTGCAGGCTGGTTATTTC-3′	This article	N/A
Primer for Abi2 forward 5′-CCGATTACTGCGAGAACAACTA-3′	This article	N/A
Primer for Abi2 reverse 5′-AGATAGGCAACACTTGCTAAGG-3′	This article	N/A
Primer for B2M forward 5′-TGCTGTCTCCATGTTTGATGTATCT-3′	This article	N/A
Primer for B2M reverse 5′-TCTCTGCTCCCCACCTCTAAGT-3′	This article	N/A
Recombinant DNA
pLenti-puro	Addgene	#39481
pEGFP-Abi1	Ryu et al. (2009) and this article	N/A
pdsRed-Jak2	This article	N/A
pCR2.1	Invitrogen	450045/1416345
Software and algorithms
Leica DMI 6000 software	Leica	http://microscopy.arizona.edu/sites/default/files/sites/default/files/upload/Leica_DMI6000_manual.pdf
GE IQTL software	GE	http://www.hhmi.umbc.edu/downloads/Imaging%20support%20GE/IQ%20TL%20collateral/IQTL_UserManual%208.pdf
Fuji Multi Gauge Software	Fuji	https://www.ualberta.ca/biological-sciences/media-library/mbsu/fla-5000/mulitgauge20.pdf
Prism	Graphpad	https://www.graphpad.com/scientific-software/prism/
Other
Confocal microscopy LSM 880 with Airyscan	Zeiss	RRID:SCR_020925
Leica TCS SPE	Leica	RRID:SCR_002140
Microscope Leica MDI6000	Leica	RRID:SCR_008960
GloMax Multi Detection System	Promega	RRID:SCR_015575