Figure 3.

25HC is a senolytic in multiple muscle cell types

(A) Targets, compounds, and mechanisms.

(B) Timeline of cell viability assay with inhibitors. Cells were pre-cultured for 24 h, treated with Doxo for 24 h, and then treated with inhibitors. Cell viability was analyzed from day 0–7.

(C–E) Effects of inhibitors on viability of FAPs, SCs, and HSMMs. Cells were treated with inhibitors at the indicated concentrations. Cell viability was analyzed on day 3 (HSMMs) or 5 (FAPs and SCs). Blue and red dots indicate non-senescent (NS) and senescent (SEN), respectively. The average value at 0 μM for each group was set at 100% cell viability. p values versus NS were obtained using unpaired two-tailed Student’s t test. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.