Figure 9.

25HC is an in vivo senolytic in tissues of aged mice

(A) Timeline of 25HC treatment in aged mice. Young (5 months old) and old (24–26 months old) mice were treated with 25HC or vehicle for 5 consecutive days. (B) Mass of gastrocnemius muscles in male and female mice. Muscle weight was normalized to body weight. (C) Cdkn1a and Cryab mRNA levels in gastrocnemius (Gastroc) and soleus (Sol) muscles. RNA was collected at 7–9 d after the first treatment with 25HC or vehicle. mRNA levels of the indicated genes were quantified by qRT-PCR, and normalized to those of actin mRNA. The average value of young-vehicle was set at 1. (D) 15-d-PGJ2 analysis in urine. Urine was collected 12 h after the first treatment with 25HC or vehicle. p values were obtained using Tukey's multiple comparisons test. ∗p< 0.05, ∗∗p< 0.01, ∗∗∗p< 0.001∗∗∗∗p< 0.0001.