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. 2022 Feb 9;51:102261. doi: 10.1016/j.redox.2022.102261

Fig. 3.

Fig. 3

REV-ERBα deficiency decreases RPE phagocytic activity. (A) RPE/choroid flat mounts from 6-month-old Rev-erbα+/+ and Rev-erbα−/− mice were stained with peanut agglutinin (magenta), phalloidin (green) and DAPI (blue). Scale bar: 20 μm. (B) Quantification of engulfed cone photoreceptors per RPE cell in Rev-erbα+/+ and Rev-erbα−/− eyes. (C) Mouse primary RPE cells isolated from 8-week-old Rev-erbα+/+ and Rev-erbα−/− mice were challenged with 10 FITC-labeled porcine photoreceptor outer segments (POS) per RPE cell for 2 h at 3 weeks after seeding. POS (green), β-Catenin (magenta), DAPI (blue). Scale bar: 5 μm. (D) Quantification of engulfed POS in Rev-erbα+/+ and Rev-erbα−/− RPE cells after 2 h of treatment. (E) Mouse primary RPE cells isolated from 8-week-old Rev-erbα+/+ and Rev-erbα−/− mice were challenged with 10 microspheres (diameter of 1 μm) per cell for 6 h at 3 weeks after seeding. Microsphere (magenta); β-Catenin (green), DAPI (blue). Scale bar: 5 μm. (F) Quantification of engulfed microspheres/cell by Rev-erbα+/+ and Rev-erbα−/− RPE cells after 6 h of treatment. ***: P < 0.001, ****: P < 0.0001. Error bars indicate SD. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)