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. 2022 Feb 9;119(7):e2115799119. doi: 10.1073/pnas.2115799119

Fig. 3.

Fig. 3.

EBP50 regulates ezrin expression and localization. (A) Influence of EBP50 on ezrin expression. Wild-type (WT), EBP50 knockout (KO), and ezrin knockdown (KD) HEK293T cells were subjected to immunoblot with anti-EBP50, anti-ezrin and anti-GAPDH antibodies (Left). Densitometry reported as mean + SD, n = 3; *P < 0.05; ns, not significant (Center, Right). (B) Immunofluorescence analysis of cells in A using anti-EBP50 and anti-ezrin antibodies (Upper). Line plots showing ezrin localization in WT and EBP50 knockout cells (n = 10 cells, Lower, Left two panels). Bar graph reports peak maximum fluorescence intensities at the membrane-proximal regions; mean + SD; n = 10 cells; ****P < 0.001 (Lower, Right).