Fig. 6.
ArSS1 causes concentration-dependent contraction of tube foot, apical muscle, and cardiac stomach preparations from A. rubens. (A) Representative recordings showing that ACh (black) and ArSS1 (red) cause contraction of a tube foot preparation when tested at a concentration of 10−6 M. The upward-pointing arrowheads show when test agents were added, and the downward-pointing arrowheads show when the preparation was washed with seawater. (B) Graph showing that ArSS1 causes concentration-dependent contraction of tube foot preparations at concentrations ranging from 10−8 M to 10−6 M. The responses are expressed as the mean percentage (± SEM; n = 8) of the contraction induced by 10−6 M ACh. (C) Representative recordings showing that ACh (black) and ArSS1 (red) cause contraction of an apical muscle preparation when tested at a concentration of 10−6 M. The upward-pointing arrowheads show when test agents were added, and the downward-pointing arrowheads show when the preparation was washed with seawater. (D) Graph showing that ArSS1 causes concentration-dependent contraction of apical muscle preparations at concentrations ranging from 10−8 M to 10−6 M. The responses are expressed as the mean percentage (± SEM; n = 8) of the contraction induced by 10−6 M ACh. (E) Representative recordings showing that NGFFYamide (10−7 M; black) and ArSS1 (10−6 M; red) cause contraction of a cardiac stomach preparation. The upward-pointing arrowheads show when test agents were added, and the downward-pointing arrowheads show when the preparation was washed with seawater. (F) Graph showing that ArSS1 causes concentration-dependent contraction of cardiac stomach preparations at concentrations ranging from 10−9 M to 10−6 M. The responses are expressed as the mean percentage (± SEM; n = 8) of the contraction induced by 10−7 M NGFFYamide.