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. 2022 Feb 7;119(7):e2112630119. doi: 10.1073/pnas.2112630119

Fig. 3.

Fig. 3.

Mg1a is a potent ligand of mammalian ErbB1. (A) Ligand-induced BRET ratio of HEK293T cells transiently expressing hErbB1/Rluc8 and Grb2/Venus following stimulation with 100 nM rMg1a or hEGF. The arrow indicates the time of ligand addition. Data are mean ± SEM (n = 3). (B) Concentration–response curves of ligand-induced BRET ratio for HEK293T cells transiently expressing hErbB1/Rluc8 and Grb2/Venus following stimulation with rMg1a or hEGF. Data are mean ± SEM (n = 3). AUC, area under the curve. (C) Representative Western blot showing phosphorylated Erk1 and Erk2 (red) and the control housekeeping protein GAPDH (green) after stimulation of HEK293 cells transiently expressing hErbB1 with 2.5 or 10 nM rMg1a or hEGF. (D) Quantitative Western blot data showing relative intensity over the GAPDH loading control. Data are expressed as mean ± SEM (n = 3). **P < 0.01, ****P < 0.0001.