Fig. 4.

Discrete regions of gravin-α mediate associations with the β₃-AR and HDAC11. (A) Co-IP of endogenous gravin-α with endogenous β₃-AR and HDAC11 in homogenates from 3T3-L1 adipocytes. IP, immunoprecipitation; IB, immunoblotting. (B) Schematic representation of the N- and carboxyl-terminal truncation constructs of FLAG-tagged rat gravin-α that were employed for co-IP studies. (C) Schematic depiction of the experiment to map the β₃-AR binding domain on gravin-α. (D) Immunoblot analysis of ectopically expressed β₃-AR coimmunoprecipitating with the indicated gravin-α constructs in HEK293 cell homogenates. (E) Schematic depiction of the experiment to map the HDAC11 binding domain on gravin-α. (F) Immunoblot analysis of ectopically expressed HDAC11 coimmunoprecipitating with the indicated gravin-α constructs in HEK293 cell homogenates. (G) Schematic representation of gravin-α incorporating the β₃-AR and HDAC11 binding domains. Please visit Figshare for a higher-resolution version (10.6084/m9.figshare.19082813).