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. 2022 Feb 11;119(7):e2119678119. doi: 10.1073/pnas.2119678119

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Copyright © 2022 the Author(s). Published by PNAS.

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Fig. 5. — Lysine myristoylation of gravin-α is required for β₃-AR–mediated UCP1 induction. (A) Schematic depiction of the experiment to determine if gravin-α and its myristoylation are required for β₃-AR signaling in adipocytes. (B) Immunoblot analysis of UCP1 induction and gravin-α knockdown in 3T3-L1 adipocytes. α-Tubulin (α-Tub) served as a loading control. (C) Immunoblot analysis of 3T3-L1 homogenates using an anti-phospho-PKA substrates antibody. (D) Immunoblot analysis of UCP1 induction and gravin-α knockdown in HIB1B adipocytes. α-Tub served as a loading control. (E) Immunoblot analysis of HIB1B homogenates using an anti-phospho-PKA substrates antibody. Please visit Figshare for a higher-resolution version (10.6084/m9.figshare.19082813).