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ΔtrxA OMVs associated cytotoxicity is protein-dependent. (a) J774 macrophage-like cells were incubated with WT or ΔtrxA OMVs (containing 1 or 10 µg protein) for 1 or 4 h, and stained with AO/EB to determine cell death (percentage). (b) J774 cells were incubated with WT or ΔtrxA OMVs (10 μg/well) in the presence or absence of protease K (PK, 10 μg/well) for 4 h, and cell viability was determined by AO/EB staining. Five samples per group were analyzed. *P < 0.05, t-test.
