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. 2022 Feb 7;119(7):e2120404119. doi: 10.1073/pnas.2120404119

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Copyright © 2022 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY).

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Fig. 4. — Conformational changes of TRPML1 between the PI(3,5)P₂-bound closed and PI(3,5)P₂/Tem-bound open states. (A) Structural comparison of TRPML1 between the PI(3,5)P₂-bound closed (magenta) and PI(3,5)P₂/Tem-bound open (green) states. (B) Conformational changes at the Tem-binding region. Arrows indicate the Tem-induced movements at S5 and S4 from the neighboring subunit (labeled as S4′). (C) Two subtle movements at the PI(3,5)P₂-binding region. Arrows indicate the movements from the closed to open state. (D) Zoomed-in view of the R403/Y404-mediated bending movement of S4. (E) Zoomed-in view of R403/Y404-mediated interactions that are observed in the open state (Right) but absent in the closed state (Left). (F) Top view of the pore-opening movement induced by the outward movement of the S4 C terminus. (G) Top view of the counterclockwise rotation of the luminal linker domain from the closed to open state.