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. Author manuscript; available in PMC: 2022 Feb 17.
Published in final edited form as: J Leukoc Biol. 2020 Sep 18;109(4):793–806. doi: 10.1002/JLB.3A0320-210R

FIGURE 4. Effect of EVs on endothelial cells.

FIGURE 4

HUVEC were pretreated for 6 h (E-Selectin) or 24 h (VCAM-1 and IL-8) with 1 of the 3 EV populations or their controls. IL-8 amount of the supernatant was quantified with ELISA (A and D). E-Selectin and VCAM-1 expression was determined by cellular ELISA (B, C, E, and F). Panels (A)–(C) show the summarized changes in IL-8 secretion, E-Selectin, and VCAM-1 expression of the EV-treated cells. Panels (D)–(F) show the normalized data pairs for aEV or control-treated cells from each experiment. Data were normalized to their adequate controls (“aEV” to “Lysed aEV”, “sEV” and “apoEV” to “No EV”). Raw data were compared using paired Student’s t-test; n = 5. Error bars represent mean + SEM