Fig. 3.

Expression and correlation of miR-486-5p and ANK1 before and after 5-Aza-2′-deoxycytidine treatment in osteosarcoma cell lines. A. Quantification of expression of miR-486-5p and ANK1 versions 1–4 in untreated cells. B. The induction of miR-486-5p and ANK1 versions 1–4 after 5-Aza treatment. The induction fold change is calculated as the ratio between expression of untreated and 5-Aza treated transcripts as quantified by qRT-PCR. The expression is normalized against RNU44 for miR-486-5p and GAPDH for ANK1. Pearson’s Correlation r is calculated with and without the outlier sample MG-63