REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
PE anti-human HLA A2 (clone BB7.2)	BioLegend	Cat#343305;RRID:AB_1877228
FITC anti-human HLA-A2 (clone BB7.2)	BioLegend	Cat#343303;RRID:AB_1659246
PerCP labelled human CD8+ (clone SK1)	BioLegend	Cat#344708;RRID:AB_1967149
APC labelled human CD8+ (clone T8)	BioLegend	Cat#301049;RRID:AB_2562054
APC labelled human CCR7 (clone G043H7 )	BioLegend	Cat#353212;RRID:AB_10916390
FITC labelled human CD45RA (clone HI100)	BioLegend	Cat#304150; RRID:AB_2564158
anti-human CD28 Antibody (clone CD28.2)	BioLegend	Cat#302901; RRID:AB_314303
APC anti-human CD69 (clone FN50)	BioLegend	Cat#310909;RRID:AB_314844
APC Annexin V	BioLegend	Cat#640919
PerCP anti-human IFN-γ (clone 4S.B3)	BioLegend	Cat#502524;RRID:AB_2616613
FITC anti-human Granzyme B (clone GB11)	BioLegend	Cat#515403;RRID:AB_2114575
Biological samples
Blood samples from healthy donors, COVID-19 convalescent	Guangzhou Blood Center, Guangzhou Center for Disease Control and Prevention	N/A
Chemicals, peptides, and recombinant proteins
Lymphocyte separation medium	GE	Cat#MQ0148
Fetal bovine serum	LONSERA	Cat#S711-001S
Dimethyl sulfoxide	Sigma-Aldrich	Cat#D2650
MHC monomer	BioLegend	Cat#280003
PE streptavidin	BioLegend	Cat#405203
Mitomycin C	sinochem	Cat#50-07-7
CFSE	TargetMol	Cat#T6802
IL-2	SL PHARM	N/A
GolgiPlug	BD Biosciences	Cat#550583
50 mM biotin	Invitrogen	Cat#2110450
Critical commercial assays
pMHC ELISA Kit	Mlbio	Cat#1269746
EasySep Human negative selection CD8 T	Stemcell	Cat#17953
Experimental models: Cell lines
T2-A2	Dr. Anna Gil	N/A
Software and algorithms
FlowJo software version 10.7	FlowJo LLC	https://www.flowjo.com/
Prism version 8	Graphpad	https://www.graphpad.com/
SPSS Statistics 22	IBM	https://www.ibm.com/
Other
Each epitope corresponds to a tetramer	Home-made	N/A