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. 2022 Feb 16;20:49. doi: 10.1186/s12915-022-01250-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Coding regions of ccmFc1 and ccmFc2 expanded after breaking. a Position and direction of primers and agarose gel of cDNA confirming gene expansion. Gene lengths referred to sweet potato. Lanes 1 to 7 represent Cu. australis, Cu. japonica, sweet potato, Dichondra, tomato, Arabidopsis, and no template. b Peptides (shown by short green segments) were identified by mass spectrometry data. c Independent nonsense mutations truncated the expansion of ccmFc1 amino acid sequences in Cuscuta. Blue lines indicated the position of F1 and R3 primers. Red lines under the sequence showed coding regions. The vertical black dotted line indicated the breakpoint. d Transmembrane helices predicted by TMHMM: top, tomato ccmFc; middle, sweet potato ccmFc1; bottom, sweet potato ccmFc2