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. 2022 Feb 16;23:55. doi: 10.1186/s13059-022-02628-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Preferable hashing techniques according to our study. TotalSeq antibody hashing is a preferred method for multiplexing different human cell lines, PBMCs, mice spleen, lung, and skin cells. Lipid-modified oligos are better on mice brain cells. The CMO hashing method yields the best results when nuclei are the input sample. CMO cholesterol-modified oligos