High-fat-diet-induced obese mice received 1 week of injections with a GABA-T targeted or scramble control ASO (12.5 mg/kg i.p. twice weekly) before hyperinsulinemic euglycemic clamps were performed.
(A) Body weight the day of clamp procedures.
(B and C) Blood glucose concentrations (B) and glucose infusion rate during the clamps (C).
(D) Serum insulin concentrations before insulin infusion (basal) and during the clamp.
(E and F) Endogenous glucose appearance (Ra) (E) and glucose disappearance (Rd) (F) before insulin infusion (basal) and during the clamp.
(G) Tissue specific 14C-2-deoxyglucose uptake (PR, perirenal adipose tissue; WAT, white adipose tissue).
Number below bar denotes n per group. All data are presented as mean ± SEM.