Extended Data Fig. 1. CTR1 expression in endothelial cells and characterization of inducible Endothelial CTR1-KO mice.

A. Co-staining for CTR1 and isolectin B4 (IB4) in a postnatal day (P)5 mouse retina in CTR1 WT and CTR1^iECKO mice in developmental retina angiogenesis models. Arrows indicate the tip sprouts of vessels. B. Co-staining for CTR1 and CD31 (EC marker) or Mac-3 (macrophage marker) and their colocalization (merged, white arrows) on day 3 (upper) and day 14 (lower), respectively, in ischemic gastrocnemius muscles in hindlimb ischemia models. Representative images from n=3 independent experiments are shown. C. Strategy to generate tamoxifen-inducible EC-specific CTR1 knockout (CTR1^iECKO) mice by crossing CTR1^flox/flox mice with VE-Cadherin (Cdh5)-ERT2 Cre delete mice, which specifically express Cre in ECs upon tamoxifen administration. D and E. mRNA and proteins from aortic ECs, liver and lung isolated from WT and CTR1^iECKO mice and analyzed by qPCR and Western blotting using CTR1 antibody or Actin antibody (loading control) (n=6 biologically independent cells/samples), two-tailed unpaired t-test, **p= 0.0085. F. Real time qPCR analysis of CTR1 mRNA in HUVECs transfected with control or CTR siRNAs. (n=3 biologically independent experiments), two-tailed unpaired t-test, **p= 0.0023. NS= not significant. Data are mean ± SEM). Source numerical data and unprocessed blots are available in source data.