Table 1.
Flow Cytometry antibodies and cellular markers
| Antibody | Fluorophore | Clones | Cell Marker | Company |
|---|---|---|---|---|
| BDCA2 | BV421 | V24-785 | DCs, conventional 2 | BD Biosciences |
| CD123 | BV510 | 9F5 | DCs, plasmacytoid | BD Biosciences |
| CD14 | Alexa700 | M5E2 | Monocytes CD14+CD16− | BD Biosciences |
| CD16 | PE-Cy7 | 3G8 | Monocytes | BD Biosciences |
| CD11c | PerCP-Cy5.5 | B-ly6 | All dendritic cells | BD Biosciences |
| CD8 | BUV737 | SK1 | CD8+ T cells | BD Biosciences |
| CD4 | BUV395 | SK3 | CD4+ T cells | BD Biosciences |
| CD3 | BV786 | HIT3α | T cells | BD Biosciences |
| CD66 | BV605 | B1.1/CD66 | Neutrophil | BD Biosciences |
| BDCA1 | PE | F10/21A3 | DCs, conventional 1 | BD Biosciences |
| BDCA3 | FITC | 1A4 | DCs, plasmacytoid | BD Biosciences |
| CD11b | BV711 | ICRF44 | DCs and monocytes | BD Biosciences |
| SCARF1 | Alexa647 | All cells | R&D Systems | |
| CD45 | PE CF594 | 2D1 | All lymphocytes | BD Biosciences |
| CD19 | APC Cy7 | SJ25C1 | B cells | BD Biosciences |
| STAT1 | PE | D1K9Y | All cells | Cell Signaling |
| pSTAT1 | Alexa488 | 58D6 | All cells | Cell Signaling |
| pSTAT2 | Alexa488 | D3P2P | All cells | Cell Signaling |
| STAT3 | PE | D3Z2G | All cells | Cell Signaling |
| pSTAT3 | Alexa488 | D3A7 | All cells | Cell Signaling |
| MAPKp38 | PE | D13E1 | All cells | Cell Signaling |
| pMAPKp38 | Alexa488 | 28B10 | All cells | Cell Signaling |
| ERKp44/42 | PE | 137F5 | All cells | Cell Signaling |
| pERKp44/42 | Alexa488 | E10 | All cells | Cell Signaling |