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. Author manuscript; available in PMC: 2022 Feb 17.
Published in final edited form as: Dev Neurobiol. 2021 May 30;81(5):746–757. doi: 10.1002/dneu.22829

FIGURE 1.

FIGURE 1

Ultrastructural 3D reconstruction and analysis. (a-b) Two sections from the EM stacks of the same spine. The spines are shaded in red (a) and blue (b). A synapse between the spine head and axonal above can be seen in (b). (c-d) Reconstructed triangle mesh of the same spine, from a top (c) and a side view (d). The frame of the spine shown in (a) is colored in red, and the frame of the spine shown in (b) in blue. Scale bar: 100 nm. Spine ID: Kasthuri_3316. Coordinates: 5,812, 8,354, slices: 1,203 (a) and 1,208 (b). (e) Workflow diagram, describing the algorithm